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. 2009 Apr 2;4(4):e5143. doi: 10.1371/journal.pone.0005143

Figure 2. KCNE1 mutations D76N and Ī”70 reduce IKs current density with minimal effects on activation kinetics.

Figure 2

A) rates of activation, and B) current density from CHO cells transiently expressing Myc-tagged KCNQ1 (Q1) either without KCNE1 (E1), or with wild-type FLAG-tagged E1, E1-D76N, or E1-Ī”70. Asterisks (*) indicate statistical significance to P<0.001. nā€Š=ā€Š6. C) Immunoblot showing KCNQ1 protein levels when expressed alone or co-expressed with KCNE1 (wild-type and mutant). Western blot analysis performed with mouse anti-Myc antibody (Santa Cruz) for KCNQ1, and mouse anti-FLAG antibody (Sigma) for KCNE1. Left lane in each gel shows signal from cells that are untransfected.