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. 2009 Apr 2;4(4):e5149. doi: 10.1371/journal.pone.0005149

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Walshe et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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(A) Assay of tight junction components in Transwell cocultures of EC and 10T1/2 cells. Immunoprecipitation of occludin and western blotting for zo-1 revealed a significant increase in association between occludin and zo-1 in coculture compared to monocultures (* p<0.05), which was reversed by the addition of SB-431542. Non-specific IgG was used as a control for IP. (B) Assay of permeability in Transwell cocultures. Coculture of BAECs with 10T1/2 cells increased EC barrier function as measured by decreased flux of FITC-dextran from the upper to lower chamber. Addition of SB-431542 increased permeability of BAEC/10T1/2 cocultures.