Figure 2. Confirmation of 14 INK4a activators.

Results from four independent cotranfections of the indicated reporter with the indicated cDNA construct into U2OS cells are shown. Fold activation values were calculated for each experiment by normalizing luciferase levels of the indicated cDNA to the empty vector control (indicated as Vector). These values were then ranked by ratio of activation of the INK4a construct to activation of the empty luciferase reporter vector pGL3. Activators with relative activation values of two or more are shown. Homeodomain family members are in bold; error bars represent one standard deviation.