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Cocolonization affects the efficiency of fermentation. Cecal contents from 4 mice in each treatment group were assayed for NAD+, NADH acetate, butyrate and proprionate levels. Expression of Mct-1 mRNA, a monocarboxylate transporter whose preferred substrate is butyrate was defined by qRT-PCR in the proximal colon. Cecal propionate concentrations. Mean values ± SEM are plotted; n = 4–5 mice per group; ∗, P < 0.05, ∗∗, P < 0.001 compared with cocolonization (Student's t test).
