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. 2009 Apr 10;5(4):e1000370. doi: 10.1371/journal.ppat.1000370

Figure 2. Intra-cellular localization of GFP::FKTF-1b phosphorylation mutants.

Figure 2

DIC and fluorescence images of representative hypodermal cells of transgenic S. stercoralis L1s. In all images, the nucleus is identified with an arrow. All scale bars = 10 µm. (A,B) L1 expressing pPV244(S238E/T240E), the “phospho-mimetic” GFP::FKTF-1b with fluorescence in the cytoplasm. (C,D) L1 expressing pPV234, GFP::FKTF-1b(wt), with fluorescence in both the cytoplasm and the nucleus. (E,F) L1 with strong nuclear localization of the FKTF-1b(4A) fusion protein. (G) Percentages of hypodermal cells in transgenic larvae with intra-cellular localization of GFP classified as “cytoplasmic”, “cytoplasmic/nuclear”, or “nuclear”. Results include transgenic larvae from at least two separate microinjection experiments per transgene. Phospho-mimetic: n = 47 GFP+ cells in 11 larvae. GFP::FKTF-1b: n = 29 GFP+ cells in 8 larvae. Phospho-null: n = 37 GFP+ cells in 8 larvae. χ2 test P = 1.23E-10.