Fig. 3.

LPS induces cyclooxegenase-2 (COX-2) expression and prostaglandin E₂ (PGE₂) secretion in intestinal MF. Cells were treated with 20 ng/ml LPS for the indicated times or left untreated (Con). A: COX-2 expression was detected by Western blotting of total cell lysates. Actin is shown as a loading control. Blots shown are representative of 4 independent experiments. B: secreted PGE₂ was measured by ELISA in supernatants from MF treated as indicated; control cells were incubated in media alone. Data are presented as means ± SD and are representative of 4 independent experiments. *P < 0.05 vs. control. C: MF derived from LPS-resistant C3H/HeJ or wild-type syngeneic C3H/HeOUJ mice were stimulated with LPS for 24 h, and secreted PGE₂ was measured in supernatants as in B. Data show the means ± SD of duplicate samples, each assayed in duplicate. *P < 0.05 vs. control of the same cell type.