Fig. 5.

Multiple intracellular signaling pathways are induced by LPS treatment of intestinal MF. Cells were treated with 20 ng/ml LPS for the indicated times or with media alone (Con). Total cell lysates were analyzed by SDS-PAGE and Western blotting for the phosphorylated p65 subunit of NF-κB (p-p65) and Iκ-Bα to assess activation of NF-κB signaling. Actin is shown as a loading control. The same samples were also analyzed for phosphorylated Akt (p-Akt) and total Akt and for phosphorylated p38 MAPK (p-p38 MAPK) and total p38 MAPK. Blots shown are representative of 4 independent experiments.