Fig. 6.

Inhibition of intracellular signaling attenuates gene expression. Expression of IL-6 and KC mRNAs was assessed by semiquantitative RT-PCR. GAPDH is shown as a loading control. A: cells were treated with LPS in the presence of wortmannin (W), Akt inhibitor IV (A), SB239063 (S), or NF-κB-essential modulator (NEMO)-binding domain binding peptide (N). Control cells were incubated with media alone. Images shown are representative of 4 independent experiments. B: cells were infected with adenovirus expressing dominant negative IκB (AdIκB) or green fluorescent protein (GFP) (AdGFP) and treated with 20 ng/ml LPS as indicated. Images shown are representative of 2 independent experiments.