Fig. 8.

Independent activation of phosphoinositide 3 (PI3)-kinase and p38 MAPK pathways by LPS stimulation. A: cells were treated with media alone (Con), LPS alone (LPS), or in the presence of wortmannin (LPS + W), Akt inhibitor IV (LPS + A), or SB239063 (LPS + SB). Total cell lysates were analyzed in duplicate by SDS-PAGE and Western blotting with antibodies as described in Fig. 5. Blots are representative of 1–3 independent experiments. Actin is included as a control for protein loading for the phospho-NF-κB p65 and Iκ-Bα blots, whereas total Akt and total p38 MAPK blots are shown to demonstrate protein loading for the p-Akt and p-p38 MAPK blots, respectively. B: MF were treated with LPS alone or in the presence of Akt inhibitor IV, SB239063, or both inhibitors. Supernatants were collected after 24 h and analyzed by ELISA for IL-6 and KC. Data represent the means ± SD of duplicate samples, each assayed in duplicate, and are representative of 3 independent experiments.