Fig. 11.

Control mice and HIF-1α-deficient mice were subjected to bile duct ligation or sham operation. Fourteen days later, α-smooth muscle actin levels were quantified by real-time PCR and immunohistochemistry. A: quantification of α-smooth muscle actin mRNA levels in the livers of mice. ^aSignificantly different from sham-operated mice (P < 0.05). ^bSignificantly different from control mice subjected to bile duct ligation (P < 0.05). Data are expressed as means ± SE; n = 6. Representative photomicrograph of a section of liver from a bile duct-ligated control mouse (B) and HIF-1α-deficient mouse (C) stained for α-smooth muscle actin. Positive staining for α-smooth muscle actin appears dark gray in the photomicrographs.