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Livers were obtained from HIF-1αfl/fl-Mx-Cre+ mice and HIF-1αfl/fl-Mx-Cre− mice treated with polyinosinic-polycytidylic acid. PCR was used to estimate the extent of HIF-1α deletion in the livers. The floxed, undeleted HIF-1α gene produces a 441-bp PCR fragment, whereas the floxed, deleted HIF-1α gene produces a 355-bp PCR fragment.
