Fig. 1.

Effect of hypoxia and hypoxia-reoxygenation (H/R) on Bcl-2/adenovirus EIB 19-kDa interacting protein 3 (BNIP3) expression in hepatocytes. Freshly isolated rat and mouse hepatocytes were exposed to hypoxia (H; 1% O₂) for 6 h with or without reoxygenation for 18 h, and the mRNA and protein were isolated and analyzed as described in materials and methods. Control samples were hepatocytes cultured under normoxic (i.e., nonischemia) conditions (N). A: Northern blot analysis for BNIP3 mRNA in rat hepatocytes. A representative blot (inset) and the densitometric quantification of 5 independent experiments are shown. *P = 0.01 vs. normoxia. Data were analyzed by Student's t-test. B: Western blot analysis for BNIP3 protein in mouse hepatocytes. A representative blot (inset) and the densitometric quantification of 5 independent experiments are shown. *P < 0.05 vs. normoxia and H/R. Data were analyzed by 1-way ANOVA followed by Student-Newman-Keuls test. Reox, reoxygenation. C: lactate dehydrogenase (LDH) release in mouse hepatocytes exposed to hypoxia or H/R. Results are means ± SE (n = 9–16). *P < 0.001 vs. normoxia. **P < 0.05 vs. hypoxia. Data were analyzed by 1-way ANOVA followed by Tukey's test.