Western blot for PKC-α (A), CPI-17 (B), and Phospho-CPI-17 (C). PKC-α was significantly elevated in SM-B KO bladders (8.87 ± 0.34) ODUs compared with WT bladders (5.0 ± 0.43) ODUs, P < 0.05. There was no difference in the total CPI-17 level (B); however, the level of phospho-CPI-17 (C) was higher in the SM-B KO mice (5.04 ± 0.95) compared with the WT mice (2.74 ± 0.78) ODUs, n = 4, P < 0.05. D: results for ROKα, ROKβ, and RhoA. The data reveal no significant difference between WT and SM-B KO bladders.