Fig. 10.

Levels of lipid peroxidation (A), H₂O₂ production (B), cell death (C), flow cytometric analysis of annexin V-FITC/PI staining (D), and numbers of apoptotic cells (E) and necrotic cells (F) in LLC-PK₁ cells transfected with LNCX-IDPc, LNCX-null, IDPc-siRNA, or scrambled siRNA 2 h after 1 mM H₂O₂ treatment. LLC-PK₁ cells were treated with either 1 mM H₂O₂ or vehicle. Two hours after treatments, levels of lipid peroxidation (A) and H₂O₂ production (B) and cell death (C) were determined as described in materials and methods. Apoptotic cell (E) and necrotic cell (F) numbers were determined by flow cytometric analysis using annexin V-FITC positive/PI-negative cells and annexin V-FITC positive/PI-positive cells, respectively (D). Results are means ± SE (n = 6). *P < 0.05 vs. respective vehicle.