Fig. 2.

Expressions of IDPc in mouse kidney tubular epithelial cells. Kidney sections were immunostained with antibodies described in materials and methods. A–C: kidney sections were stained with anti-IDPc antibody. D–I: kidney sections were double-stained with anti-IDPc and then with anti-Na⁺-K⁺-ATPase or anti-gp330 antibodies. Pictures were taken of the cortex. IDPc is highly expressed in the cytoplasm of proximal tubule (PT, *) cells but weakly in distal tubule (DT, #) cells. J–L: serial kidney sections were mounted on the same slide for staining with anti-IDPc, anti-Na⁺-K⁺-ATPase, or anti-Na⁺-K⁺-Cl⁻ cotransporter 2 (NKCC2) antibodies. Pictures were taken of the inner strip of the OM. IDPc expression is weak in the cytoplasm of tubules. *, Thick ascending limb (TAL). M–R: kidney sections were double-stained with anti-IDPc antibody and then with either anti-Na⁺-K⁺-ATPase or anti-gp330 antibodies. S1–2 segment cells of the PT in the cortex express IDPc to a much greater extent than S3 segment cells in the outer strip of the OM. *, S3 segment of the PT. S and T: serial kidney sections were stained with anti-IDPc and anti-AQP1 antibodies. Pictures were taken of the OM. Descending thin limb (DTL) cells faintly express IDPc in the cytoplasm. *, DTL in the OM (omDTL). U and V: serial kidney sections were stained with anti-IDPc and anti-AQP1 antibodies. Pictures were taken of the IM. Insets in A, D, S: kidney treated with secondary antibody alone. *, DTL in the IM (imDTL). D–I and M–R: pictures taken of the double staining merged with Photoshop software. Scale bars: 500 (A–C) and 50 (D–V) μm.