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. 2008 Dec 23;296(3):F622–F633. doi: 10.1152/ajprenal.90566.2008

Fig. 5.

Fig. 5.

Expression (A and B) and activity (C) of IDPc, ratio of NADPH to total NADP (NADPH/NADPt; D), ratio of oxidized glutathione to total glutathione [GSSG/(GSH+GSSG); E], and level of total glutathione (F) in whole kidneys of mice subjected to either 30 min of bilateral ischemia or sham operation. Kidneys were harvested 4 and 24 h after reperfusion. A: IDPc expression was determined by Western blot analysis using anti-IDPc antibody. Antibody against β-actin was used as an equal loading marker. B: densities of blots were quantified with the Lab Works program (n = 4). IDPc activity (C), NADPH/NADPt (D), GSSG/(GSH+GSSG) (E), and the level of total glutathione (F) were determined in the kidneys as described in materials and methods. Results are means ± SE (n = 6). OD, optical density.