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Levels of superoxide (A), H2O2 (B), and lipid peroxidation (C) in kidneys of mice subjected to either 30 min of bilateral ischemia or sham operation. Kidneys were harvested 4 and 24 h after surgery. A: superoxide formation was determined by conversion of nitro blue tetrazolium (NBT) to formazan (dark brown color). Levels of H2O2 (B) and lipid peroxidation (C) were determined in kidneys as described in materials and methods. Results are means ± SE (n = 6).
