Fig. 10.

Immunoblot and Northern blot analysis for Rhcg in C57/Bl6 and Balb/c mouse kidneys. A: immunoblot assay for Rhcg in kidney proteins from C57BL/6 and Balb/c mice. There is no detectable difference in Rhcg protein molecular weight between C57BL/6 and Balb/c kidneys, suggesting that the molecular weight of basolateral Rhcg is not detectably different from apical Rhcg. B: Northern blot analysis of total RNA from C57BL/6 and Balb/c mouse kidneys using 2 different probes directed against nucleotides 371–763 and 763-1507 of mouse Rhcg DNA. GAPDH is used as an internal control for loading and transfer efficiency. Single bands for Rhcg mRNA at ∼2.1 kb are present in both mouse strains. Results in each panel are representative of findings from at least 3 animals of each strain.