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. 2009 Jan 7;296(3):F543–F555. doi: 10.1152/ajprenal.90637.2008

Fig. 3.

Fig. 3.

Double immunolabeling for Rhcg and aquaporin-2 (AQP2) in the C57BL/6 mouse kidney. Double immunolabeling for Rhcg (brown) and AQP2 (blue) was performed to confirm that both intercalated and principal cells express basolateral Rhcg immunoreactivity. A: CCD demonstrating that both principal cells (black arrows) and intercalated cells (white arrows) express basolateral Rhcg immunolabel. B: OMCD and confirming the presence of basolateral Rhcg immunolabel in both principal cells (black arrows) and intercalated cells (white arrows). C: representative IMCD. Intercalated cells (white arrows), identified by the absence of AQP2 immunoreactivity, express both apical and basolateral Rhcg immunolabel. IMCD cells (black arrows) do not express detectable Rhcg immunolabel. Basolateral AQP2 immunolabel in the IMCD has been demonstrated previously by others (22). Rhcg antibody A was used in these studies. Results are representative of findings in at least 6 mice.