Fig. 8.
Demonstration of kidney-specific genomic recombination of Rhcg gene in Rhcgfl/fl, Ksp-cadherin-Cre positive mice. PCR amplification of genomic DNA from kidneys of Ksp-cadherin-Cre-positive, Rhcgfl/fl mice yields a product of 750 bp, the predicted size of the product following recombination at the loxP sites flanking exons 5–9 of the Rhcg gene. A lesser amount of a 3.6-kb product is present, indicating that recombination was not 100%. PCR amplification of tail DNA from the same animals yields only the 3.6-kb product, the predicted size in the absence of genomic recombination. PCR amplification of kidney and tail-clip DNA from Rhcgfl/fl littermates negative for Ksp-cadherin-Cre yields a product of 3.6 kb, indicating no genomic recombination in the absence of Ksp-cadherin-Cre expression.