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Renal cAMP levels in PCK rats following treatment with 20-HETE synthesis inhibitor. Protein lysates were extracted by 0.1 N HCl from PCK rat kidneys treated with vehicle (n = 7) or HET-0016 (n = 3), and the intracellular levels of the second messenger cAMP was measured by ELISA. *P < 0.05 significant difference between groups.
