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Immunoblot analysis of ERK1/2 and Stat3 in PCK rats. Protein lysates from the PCK rats treated with vehicle (VEH) or HET-0016 (HET) were electrophoresed and immunoblotted with primary antibodies targeted to phospho-ERK1/2 (p44/42 MAPK; A), total ERK1/2 (p44/42 MAPK; B), Stat3 (C), and GAPDH (D); n = 3 kidney samples/group.
