Table 3.
Primers used for monoplex PCR and multiplex PCR
| Factor class |
Gene | Primer (pmol/μL) |
Primer sequence (5′-3′) | PCR product in bp a |
Accession no.b |
Annealing temp |
Reference |
|---|---|---|---|---|---|---|---|
| SPATE- gene |
sat | 25 | TCAGAAGCTCAGCGAATCATTG CCATTATCACCAGTAAAACGCACC |
930 | AE014075 | 59 °C | This study |
| sigA | 25 | CCGACTTCTCACTTTCTCCCG CCATCCAGCTGCATAGTGTTTG |
430 | NC_004337 | 58 °C | This study | |
| pet | 25 | GGCACAGAATAAAGGGGTGTTT CCTCTTGTTTCCACGACATAC |
302 | AF056581 | 58 °C | 44 | |
| pic | 25 | ACTGGATCTTAAGGCTCAGGAT GACTTAATGTCACTGTTCAGCG |
572 | AF097644 | 58 °C | 44 | |
| sepA | 25 | GCAGTGGAAATATGATGCGGC TTGTTCAGATCGGAGAAGAACG |
794 | Z48219 | 58 °C | 44 | |
| tsh | 25 | CCGTACACAAATACGACGG GGATGCCCCTGCAGCGT |
900 | AF218073 | 59 °C | 44 | |
| vat | 25 | AACGGTTGGTGGCAACAATCC AGCCCTGTAGAATGGCGAGTA |
420 | AY151282 | 58 °C | 44 | |
| eatA | 25 | CAGGAGTGGGAACATTAAGTCA CGTACGCCTTTGATTTCAGGAT |
743 | AY163491 | 60 °C | 44 | |
| espP | 25 | GTCCATGCAGGGACATGCCA TCACATCAGCACCGTTCTCTAT |
547 | NC_002128 | 60 °C | 44 | |
| espC | 25 | TAGTGCAGTGCAGAAAGCAGTT AGTTTTCCTGTTGCTGTATGCC |
839 | AF297061 | 59 °C | 44 | |
| AAF gene |
aggA | 25 | AAATATGAGAAGAAAGAA AAAAATTAATTCCGGTATGG |
500 | ECU12894 | 50 °C | 41 |
| aafA | 25 | CAGAATGTTTGCGATTGCTAC TTTGTCACAAGCTCAGCATT |
468 | AF012835 | 50 °C | 41 | |
| agg3A | 25 | GTATCATTGCGAGTCTGGTATTCAG GGGCTGTTATAGAGTAACTTCCAG |
462 | AF411067 | 50 °C | 45 | |
| agg4A | 25 | TCCATTATGTCAGGCTGCAA GGCGTTAACGTCTGATTTCC |
411 | EU637023 | 59 °C | 41 | |
a
bp, Base pair
b
GenBank database accession number for the SPATE and fimbrial genes