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. 2009 Jan 9;296(3):L547–L554. doi: 10.1152/ajplung.90333.2008

Fig. 4.

Fig. 4.

Interaction between eNOS and caveolin-1 (A), calmodulin (B), and Hsp90 (C) in pulmonary arteries isolated from control (normoxia) and prenatal hypoxia groups. Tissue lysates were immunoprecipitated (IP) with anti-eNOS antibody and immunoblotted (IB) with antibodies recognizing caveolin-1, calmodulin, and Hsp90. To ensure equal loading in each lane, blots were reprobed with anti-eNOS antibody. The extent of caveolin-1, calmodulin, and Hsp90 associated with eNOS was quantified by scanning densitometry and normalized to eNOS. Values are means ± SE; n = 5 for each group. **P < 0.01, significantly different from intrapulmonary arteries from normoxic control.