Fig. 3.

Representative Western blots (A) and summary densitometry data (B) of total protein expression of endothelial nitric oxide synthase (eNOS), phospho-eNOS (p-eNOS; Ser¹¹⁷⁷), heat shock protein 90 (Hsp90), Akt, phospho-Akt, and caveolin-1 (Cav-1) in isolated PRA from the 3 age groups. Total eNOS expression was greater in PRA from 2-day-old piglets compared with the older age groups. No significant differences in total protein expression for any other protein were detected when summary densitometry data of total protein-to-actin ratios were subjected to statistical analysis (*P = 0.002, 2-day-old different from 2 older age groups; n = 9 piglets in each age group).