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. 2009 Jan 9;296(3):L555–L564. doi: 10.1152/ajplung.90456.2008

Fig. 8.

Fig. 8.

A–C: eNOS-Hsp90 coimmunoprecipitation in PRA from 2-day-, 5- to 7-day-, and 12-day-old piglets. PRA homogenates were immunoprecipitated with anti-eNOS antibody, and the resultant immunoprecipitate was subjected to immunoblot analysis using antibodies against eNOS and Hsp90. ACh (10 μM × 1 min) stimulated eNOS-Hsp90 coimmunoprecipitation in 5- to 7-day- and 12-day-old PRA, whereas ACh fails to enhance the coimmunoprecipitation of Hsp90 and eNOS in 2-day-old PRA. Geldanamycin (GA; 1 μM × 20 min) pretreatment prevents the ACh-stimulated increase in Hsp90-eNOS binding in the older 2 age groups. n = PRA from 10 2-day-old piglets, 8 5-day-old to 7-day-old piglets, and 8 12-day-old piglets; *P < 0.05, different from Vehicle control (DMSO).