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Anti-TGF-β1 antibodies inhibit hypoxic Akt phosphorylation (phospho-Akt). HPASMC were exposed to 1% or 21% FiO2 for 4 or 8 h with and without anti-TGF-β antibody (50 ng/ml). Membranes were probed with antibodies raised against phosphorylated Ser473 (S473). To confirm equal protein loading, the blot was stripped and probed for total Akt with anti-Akt antibody.
