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Hypoxia induces NOX4 gene expression. HPASMC were exposed to 1% O2 for 24 h, and total RNA was isolated. Real-time PCR of reverse-transcribed mRNA was performed using NOX4 primers. Results were normalized to polymerase II (Pol2) and expressed as fold induction over control ± SD. *P < 0.05, 1% vs. 21% O2.
