Fig. 1.
Shear stress decreases PKCδ activation in ovine pulmonary arterial endothelial cells (PAECs). Ovine PAECs were exposed or not to shear stress (20 dyn/cm2, 8 h) and then whole cell extracts, membrane (M), and cytosolic (C) fractions were prepared by differential centrifugation. Extracts (20 μg) were then subjected to Western blot analysis using either a PKCδ antibody (A and C) or a phospho-specific antibody recognizing Tyr 311 (the activation site of PKCδ; C). C: whole cell extract data only. Shear stress decreases both PKCδ membrane translocation (B) and phopsho-Tyr 311 levels (D), indicating that PKCδ activity is diminished by shear stress. Data are means ± SD. *P < 0.05 vs. static; n = 3–4.