Fig. 3.
Effect of modulating PKCδ activity on eNOS protein levels and nitric oxide (NO) production in response to shear stress. Ovine PAECs were transfected with DN PKCδ, then treated or not with bryostatin (10 ng/ml for 30 min), and then exposed to shear (20 dyn/cm2, 8 h). Extracts (20 μg) were then subjected to Western blot analysis using a specific antibody raised against eNOS. A: representative image. DN PKCδ significantly increases, while bryostatin attenuates, the shear-mediated increase in eNOS protein levels (B). DN PKCδ also reverses the effect of bryostatin on eNOS protein levels (B). Similarly, DN PKCδ significantly increases, while bryostatin attenuates, the shear-mediated increase in NO generation (C). DN PKCδ also reverses the effect of bryostatin on NO levels (C). Values are means ± SD. *P < 0.05 vs. no shear; †P < 0.05 vs. shear alone; n = 3–6.