Fig. 6.

Effect of PKCδ or STAT3 inhibition on NO signaling in resting PAECs. To examine the effect of inhibiting PKCδ or STAT3 activities on eNOS promoter activity in resting PAECs, cells were trasnfected with DN PKCδ or S727A STAT3 along with the 1.6-kb eNOS promoter-luciferase reporter construct (along with β-galactosidase as an internal control). After 24 h, the luciferase activities were determined. PKCδ inhibition significantly attenuates, while STAT3 inhibition significantly enhances, eNOS promoter activity (A). Similarly, PKCδ inhibition significantly attenuates, while STAT3 inhibition significantly enhances, NO generation (B). Values are means ± SD. *P < 0.05 vs. control; n = 3.