Fig. 3.

Effect of NaHS on phosphorylation of endothelial NO synthase (eNOS) and p38 MAPK. Human microvascular endothelial cells (HMEC-1) were incubated with NaHS (100 μM) for the indicated times, lysed, and subjected to SDS-PAGE and Western blotting for both total and phospho-eNOS (Ser¹¹⁷⁷), and total and phospho-p38 MAPK (Thr¹⁸⁰/Tyr¹⁸²), and GAPDH. A: representative blot showing results from a single experiment from among the full dataset. B: densitometric quantitation of phosphorylation of eNOS and p38 normalized to total expression of eNOS and p38, respectively. Values are means ± SE of ratios of arbitrary densitometric units of phosphorylated vs. total immunoreactivity for each enzyme, obtained by scanning bands from separate repeated experiments (eNOS, n = 6; p38, n = 5). *P < 0.001, values significantly different from time 0.