Fig. 2.

Silencing MCTs/CD147 in ARPE-19 cells does not impact integrin expression. A: RT-PCR was performed on cells to determine the effectiveness of small interfering RNA (siRNA)-mediated silencing of MCT1, MCT4, or CD147 and if there were changes in expression of nontarget mRNAs. Cells were harvested for total RNA preparation 48 h posttransfection. PCR results show that MCT1, MCT4, and CD147 siRNAs efficiently silenced their respective targets. No change in mRNA levels of β₁-integrin was observed. GAPDH and β-actin served as loading controls. B: cells were harvested for protein analysis 72 h posttransfection. Silencing MCT1 and MCT4 resulted in decreased expression of CD147, while silencing of CD147 resulted in decreased expression of MCT1 and MCT4. β₁-Integrin expression was not affected by silencing MCT1, MCT4, or CD147. β-Actin was used as a loading control. Ten micrograms of total protein were loaded per lane.