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. 2009 Mar 6;6:7. doi: 10.1186/1742-2094-6-7

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Copyright © 2009 Lin et al; licensee BioMed Central Ltd.

This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Murine microglia express CNTFRα, which is induced further by IFNγ. Panel A shows a representative photograph of cultured murine microglia. Panel B shows representative western blots for CNTFRα proteins in murine microglial cultures induced by IFNγ. Microglia were treated with IFNγ 1, 10 and 100 ng/mL, or left untouched for 22 hours. Forty micrograms of protein lysates were analyzed by western blotting. Membranes were probed with CNTFRα antibody and were re-probed with β-tubulin antibody to confirm equivalent protein loading. Recombinant rat soluble CNTFRα was loaded as a positive control (leftmost lane).