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. 2009 Mar 6;6:7. doi: 10.1186/1742-2094-6-7

Figure 2.

Figure 2

CNTF does not activate STAT or ERK pathways in murine microglia. After cytokine treatment, ten micrograms of protein lysates were analyzed by western blotting for phosphorylated proteins and the membranes were striped and reprobed with antibody against total protein. A, Enriched murine microglial cultures were treated with CNTF (10 ng/mL), IL-6 (5 ng/mL), LIF (10 ng/mL), CNTF (10 ng/mL) plus soluble CNTFRα (200 ng/mL), IL-6 (5 ng/mL) plus soluble IL-6R (200 ng/mL), or left untouched (UT) for 20 minutes. B, Murine astrocytes were treated with CNTF (10 ng/mL) or IL-6 (5 ng/mL) or left untreated for 20 minutes. C, Enriched rat microglial cultures (purity > 99%) were treated with CNTF (10 ng/mL) or IL-6 (10 ng/mL) or left untreated for 20 minutes. D, Murine microglia were treated with CNTF (10 ng/mL) for 2, 5, 20, 40 and 60 minutes or IL-6 (5 ng/mL) or left untreated for 20 minutes. Data are representative of 3 independent experiments.