Figure 3.

Effects of different kosmotropic and chaotropic salts on the refolding recovery yield. Different kosmotropes and chaotropes were added into the refolding buffer containing 400 mM Arg, 200 μM NADP⁺, and 10 mM DTT in 50 mM Tris to final concentrations of each salt at 50 and 400 mM respectively, and pH was adjusted to 8.0. The refolding mixture was incubated at 25°C and the final regained G6PD activity was followed.