Figure 2.

Partial overlap with early endosomal markers. Only partial colocalization with early endosomes of both endogenous NIPA1 protein (A–E) and expressed tagged NIPA1::EGFP protein (F–J) in cultured rat cortical neurons was seen in the neuronal bodies. We did not observe any colocalization of NIPA1 and Rab5 antigen in the NIPA1-positive vesicles of neuronal processes (E, J). There was no significant difference between the distribution of the endogenous and expressed tagged protein. Similar patterns were observed in HEK293 and COS7 cell lines for expressed NIPA1 protein (data not shown).