Figure 3.

Mislocalization of mutant NIPA1. Expressed WT NIPA1::HA demonstrated marked colocalization with the trans-Golgi complex [A, anti-HA (red); B, Golgin 245-YFP (green); C, overlay; HEK293 cells shown]. D–I, Zoom of the Golgi complex. Expressed mutant form G106R of NIPA1::HA resulted in only partial overlap with Golgi markers with additional presence of the mutant protein outside the Golgi complex (G–I). Golgi complex in cells expressing mutant forms of NIPA1 was typically thinner than in cells expressing WT NIPA1. Mutation T45R had the same effect on NIPA1 intracellular trafficking.