Table 1.
Primer sequences
| Primer | Sequence | Purpose |
|---|---|---|
| IdeR F IdeR R |
5'TTGGATCCATGAACGATCTTGTCGATAC-3' 5'-CGGAATTCTCAGACCTTCTCGACCTTG-3' |
cloning of ideR coding region into pGEX-6P-1 |
| RG329 RG330 |
5'-CCGGGATCCATGACGGGCGCGGT-3' 5'-CCGGAATTCTCACGTCTGGTTCCCG-3' |
cloning of zur coding region into pGEX-6P-1 |
| Rv0282-1 Rv0282-2 |
5'-CGGGATCCCGCAACACCCTGGTC-3' 5'-CGGGTACCCGCTGTCTCCTTCACC-3' |
EMSA on rv0282 promoter region |
| mmp3 mmp7 |
5'-GCACGCTTGAGAGTTCC-3' 5'-TGCCACTTTCGGGTC-3' |
EMSA on mmpS5 promoter region |
| Pr1MS F Pr1MS R |
5'-CCAGTACTGACGCTGGAACGAGTG-3' 5'-CCAAGCTTCTGACCACATCGCGG-3' |
EMSA and cloning of msmeg0615 promoter region into pMYT131 |
| Pr2MS F Pr2MS R |
5'-CCAGTACTACGCTGACCGGCGAC-3' 5'-CCAAGCTTCTCATGACTGTTTCCTTTC-3' |
Cloning of msmeg0620 promoter region into pMYT131 |
| Pr2MT F Pr2MT R |
5'-CCAGTACTCAACGAGCCCGAGGCG-3' 5'-CCAAGCTTCTCATAACATCTCTCC-3' |
Cloning of rv0287(esxG) promoter region into pMYT131 |
| RA1 RA2 |
5'-GACCACGCGTATCGATGTCGAC(T)16V-3' 5'-GACCACGCGTATCGATGTCGAC-3' |
5' RACE PCR reactions |
| Ms0615-RT MS0615-1 Ms0615-2 |
5'-GTCGACGACGGCCGGGGTG-3' 5'-CCGATCCACGCGTCGCAC-3' 5'-GTCGTGTGCGAGATGGGTC-3' |
5' RACE for msmeg0615 |
| Ms0620-RT Ms0620-1 Ms0620-2 |
5'-GTCGAGCAGCGCATTGAC-3' 5'-CGAGACCTCGACGAAACG-3' 5'-GCATGCGCGGCCTGGAAG-3' |
5' RACE for msmeg0620 |
| Ms0615 A Ms0615 B |
5'-GGCCTGACGGTCAACG-3' 5'-ATCCACGCGTCGCACT-3' |
qPCR for msmeg0615 |
| Ms0620 E Ms0620 F |
5'-CAGGCCGCGATGAGTT-3' 5'-TCGAGCAGCGCATTGA-3' |
qPCR for msmeg0620 |
| mysA F mysA R |
5'-CGTCGCCGATGGTCTG-3' 5'-CCACGCCCGAAGAGC-3' |
qPCR for M. smegmatis sigA (mysA) |
| Rv0282 C Rv0282 D |
5'-AGGTGTCGCGGCTGAA-3' 5'-GGTCCCGCAAACACCA-3' |
qPCR for Rv0282 |
| Rv0287 A Rv0287 B |
5'-CTGATGGCGGCACACGA-3' 5'-CAGAAACCGGGCATGG-3' |
qPCR for Rv0287 |
| sigA F2 sigA R2 |
5'-CGCGAAAAACCATCTG-3' 5'-GATCAGCCCCAGGTTG-3' |
qPCR for tuberculosis sigA |