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. 2009 Apr 3;4(4):e5071. doi: 10.1371/journal.pone.0005071

Figure 2. Association of endogenous FE65 and Teashirt proteins.

Figure 2

A, Co-precipitation of endogenous Teashirt and FE65 from rat cortical neurons. 100 µg of extract derived from rat cortical neurons were subject to immunoprecipition (IP) with either mouse IgG (mIgG) or the KD74 anti-Teashirt antibody and immunoprecipitates were subsequently immunoblotted (IB) with rabbit anti-FE65 antibody. Expression of FE65 in the extract was confirmed by immunoblotting an aliquot of extract (Input). B, Colocalization of endogenous Teashirt proteins with FE65 in cell bodies and growth cones from primary neuronal cultures. Rat cortical neurons (3.5 div) were stained with KD74 anti-Teashirt and anti-FE65 antibodies. C, Colocalization of endogenous Teashirt proteins with FE65 in H4 cells. H4 cells were immunostained with a rabbit anti-FE65 antibody and a mouse anti-Teashirt monoclonal antibody. DAPI staining was used to highlight nuclei. The upper panels show an optical section in the middle of nuclei while the lower panels show an optical section nearer to the coverslip, where there is greater cytoplasmic extension.