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. 2009 Apr 1;4(4):e5050. doi: 10.1371/journal.pone.0005050

Figure 2. Transcription regulation of heat shock response genes by NFIX, CGGBP1 and HMGN1.

Figure 2

CGGBP1 is induced by heat shock alone and depletion of HMGN1 or NFIX at 37°C. This induction by HMGN1 or NFIX depletion is further enhanced at 39°C (A). HMGN1 is induced by heat shock and depletion of CGGBP1 or NFIX at 37°C (B). NFIX is not induced by heat shock or HMGN1 depletion, but only by CGGBP1 depletion at 37°C and 39°C both (C). Bonafide heat shock response genes HSF1 (D), HSPA1A (E) and stress response gene DDIT3 (F) are induced by heat shock and all the siRNA combinations (D, E and F). Single asterisks indicate significant change compared to respective Control-37°C samples. Double asterisks indicate additional significance of change (p<0.05) due to heat shock compared to the respective samples at 37°C. The gene assayed is shown on the Y-axis and the different siRNA-temperature combinations are indicated along the X-axis. All values are average ±S.D. of ratios calculated by delta-delta Ct method using GAPDH as internal control with control-siRNA-37°C values as denominators. As for control-siRNA-37°C the value is this normalized to 1 in each case, no S.D. is shown.