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. 2009 Apr 1;4(4):e5050. doi: 10.1371/journal.pone.0005050

Figure 5. Dynamics of NFIX, CGGBP1 and HMGN1 occupancies at the HSF1 promoter in U-2987 MG cells.

Figure 5

HMGN1 occupancy is increased by CGGBP1-siRNA at 39°C (A). NFIX occupancy is increased by heat shock alone and reduced by CGGBP1-siRNA at 39°C (B). Effect of HMGN1-siRNA on NFIX occupancy mimics the effects of CGGBP1-siRNA (C). HMGN1-siRNA or heat shock does not affect CGGBP1 occupancy (D). Loss of NFIX at 39°C increases CGGBP1 occupancy (E). Loss of NFIX at 37°C increases HMGN1 occupancy (F). Single asterisks indicate significant change (p<0.05) compared to respective control-37°C samples. Double asterisks indicate additional significance of change due to heat shock compared to the respective samples at 37°C. The protein assayed in ChIP is shown besides Y-axis and the different siRNA-temperature combinations are indicated along the X-axis. All values are average ±S.D. of ratios calculated by delta-delta Ct method using the input DNA for each sample as internal control with control-siRNA-37°C values as denominators. For control-siRNA-37°C the value is this normalized to 1 in each case, so no S.D. is shown.