Figure 13. Interpolated diffusion map of eGFP in a HeLa cell.

The map presents a combination of eGFP diffusion with the associated underlying confocal image of the H2A-mRFP fluorescence. The colors correspond to the diffusion coefficient of eGFP, and each color step equals a 12.125 µm² s⁻¹ range. Regions with different diffusion times can be observed in both the cytoplasm and the nucleus, as a visual lack of correlation between the chromatin density and the diffusion times. Measurements were carried out at 37°C in 5% CO₂ atmosphere, using a water immersion objective with a NA of 1.2.