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. 2009 Apr 4;4(4):e5041. doi: 10.1371/journal.pone.0005041

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Dross et al.

This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are properly credited.

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Only the nucleus of a HeLa cell is represented on this figure and the color map shows the diffusion coefficient of eGFP in the nucleus. (A) Fluorescence intensity of H2A-mRFP1. (B) Diffusion map of eGFP (colors) underlaid by H2A-mRFP1 fluorescence intensity. The white crosses show the 48 measurement points, corresponding to a total measurement time of 288 seconds. Measurements were carried out at 37°C in 5% CO₂ atmosphere, using a water immersion objective with a NA of 1.2.