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. 2009 Apr 2;4(4):e5137. doi: 10.1371/journal.pone.0005137

Figure 4. LKB1 deletion breakpoints in cervical cancer cell lines.

Figure 4

(A) LKB1 locus (chromosome 19p13.3). (B) 140 kb region (Ensembl50; 1050000–1190000) spanning LKB1 and immediately flanking loci; intervals = 10 kb. (C) Deletion breakpoints for cell lines harboring homozygous deletions. In MS751, deleted sequences are discontinuous as shown, consistent with a more complex rearrangement. Arrows = PCR primers for HeLa specific PCR (400 bp). (D) HeLa specific PCR (400 bp) confirms presence of deletion in HeLaS3. (E) HeLa intragenic LKB1 deletion occurred in vivo. Archival blocks were cored for DNA preparation. Lanes are as follows: (−) control = no template control; tumor = metastatic adrenal deposit; non-tumor = normal adrenal (same tissue block); (+) control = HeLa cell line DNA.