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. 2009 Apr 2;4(4):e5147. doi: 10.1371/journal.pone.0005147

Figure 5. J8-specific IgG concentrations and protection in BALB/c and SCID mice following passive transfer of purified rabbit IgG.

Figure 5

Rabbits were multiply vaccinated with J8-DT and DT preparations in alum. Purified IgG (0.5 mg) was administered intraperitoneally into BALB/c and SCID mice on each of three days (day −1, 0 and +1). Controls received similar amount of normal rabbit IgG (control R-IgG). The mice were challenged on day 0 with M1 GAS and their survival monitored. (a) Demonstrates the concentration of J8-specific serum IgG in recipient BALB/c and SCID mice post intraperitoneal transfer. (b) Survival of BALB/c and SCID mice following a M1 GAS challenge (significance is represented as * where p<0.05) (c) Shows the concentration of J8-specific mouse (M) and rabbit (R) IgG in the serum of recipient mice at different time-points post antibody transfer followed by M1 GAS challenge and (d) shows survival in SCID mice following additional doses (on day 3, 5 and 8) of J8-DT R-IgG or control R-IgG post GAS challenge. The abbreviation DS1 represents dose schedule 1 (day −1, 0 and +1) whereas DS2 represents dose schedule 2 (Day −1, 0, +1, +3, +5 and 8). The mice were challenged on day 0 with M1 GAS and their survival monitored. Significance is represented as * where p<0.05.