Table 2.
Summary of physical parameters derived from fits to the biferrious DF peptide VTVH MCD data. Columns summarize parameters from both fitting methods described in the text for the DF variant and wavelength specified.
| DFsc | DFscE11D | DFscY51L | ||||
|---|---|---|---|---|---|---|
| parameter | 10,000 cm-1 | 8,000 cm-1 | 10,000 cm-1 | 7,500 cm-1 | 10,000 cm-1 | 7,020 cm-1 |
| δGS (cm-1) | 3.8 | 3.8 | 4.9 | 4.9 | 5.5 | 5.5 |
| gGS∥ | 4.0 | 4.0 | 4.0 | 4.0 | 4.5 | 4.5 |
| Asatlim | 1.29 | 1.68 | 2.03 | 2.3 | 1.03 | 1.98 |
| B-term | 2.44 | 1.03 | 1.13 | 0.03 | 8.33 | 1.63 |
| δES (cm-1) | 0.3 | 0.3 | 2.0 | 2.0 | 5.0 | 5.0 |
| gES∥ | 7.2 | 7.2 | 8.7 | 8.749 | 8.0 | 8.0 |
| Asatlim | 2.33 | 2.26 | 1.77 | 2.15 | 0.66 | 1.39 |
| B-term | 1.00 | 1.14 | 2.94 | 1.46 | 10.1 | 2.70 |
| aEES (cm-1) | 1 to 5 | 3 to 7 | 3 to 7 | 4 to 8 | 4 to 8 | 3 to 8 |
| aJ (cm-1) | -2.9 to -1.5 | -2.1 to -1.0 | -1.1 to -0.7 | -1.3 to -0.8 | -1.1 to -1.9 | -2.6 to -1.0 |
| aD1 (cm-1) | -4 to -5b | 8 to 10b | -4 to -5b | 7 to 8b | -3 to -5 | 6 to 11 |
| aE/D1 | ~0.33b | < 0.08b | ~0.33b | < 0.08b | ~0.33 | < 0.15 |
| aD2 (cm-1) | 9 to 10b | -4 to -5b | 8 to 9b | -4 to -5b | 6 to 8 | -4 to -6 |
| aE/D2 | < 0.08b | ~0.33b | < 0.08b | ~0.33b | < 0.08 | ~0.33 |
a
The ranges given were determined by systematically perturbing from the best fit value (given in insets of Figure 2) until the simulated saturation behavior was no longer within the error bars of the data sets.
b
For these values the subscripts (i.e. D1 to D2) can be interchanged and still reproduce the data