Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2009 Apr 2;4(4):e4897. doi: 10.1371/journal.pone.0004897

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

This is an open-access article distributed under the terms of the Creative Commons Public Domain declaration, which stipulates that, once placed in the public domain, this work may be freely reproduced, distributed, transmitted, modified, built upon, or otherwise used by anyone for any lawful purpose.

PMC Copyright notice

A. Validation of the Glu-Cre transgenic line with Z/AP reporter mice. H&E (panels a, c, e) and alkaline phosphatase (AP; panels b, d, f) staining of a representative Glu-Cre;Z/AP pancreas at 5 months of age. Expression of Glu-Cre localized in α-cells at the outer ring of endocrine islets, as indicated by purple AP stain. Pancreatic islets (Is) are as indicated in panel a, and images are shown at 100× (a, b) and 400× (c, d, e, f) magnification. B. Genotyping PCR using genomic DNA isolated from different cell populations of the endocrine pancreas in Glu-Cre;Vhl f/f and Vhl f/f mice at 27 months of age. Insulin-positive β-cells (I), glucagon-positive α-cells (G), and lectin-positive endothelial cells (L) were collected via flow cytometry. Negative (neg.) and positive (pos.) controls are shown next to the ladder (far left lane). Top panel shows the PCR for β-globin alleles to demonstrate the presence of genomic DNA for each cell population. Bottom panel indicates the presence of deleted Vhl alleles only in α-cells of Glu-Cre;Vhl f/f pancreas.