Figure 4. KiSS-1 and GPR54 mRNA expression in recrudescing Siberian hamster ovaries.
Semi-quantitative RT-PCR expression of A) KiSS-1 and B) GPR54 mRNA in Siberian hamsters exposed to long day (LD), or post-transfer (PT) from short to long day photoperiod for 0, 1, 2, 4, and 8 weeks. Mean ± S.E.M. relative levels mRNA expression for KiSS-1 and GPR54 shown in respective graphs. β-actin was used as a control gene for all RT-PCR reactions. Columns with different letters on bar graphs indicate p<0.05. C) Kiss-1 and D) GPR54 expression in the arcuate nucleus of Siberian hamsters, used as a positive control.