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. 2009 Apr 7;3(4):e407. doi: 10.1371/journal.pntd.0000407

Figure 4. Identification of IFN-γ secreting T cells responding to whole B. pseudomallei by four-color flow cytometry.

Figure 4

Whole blood samples from eight recovered melioidosis cases and six seropositive control subjects were incubated with whole B. pseudomallei for 12 hours and stained for intracellular IFN-γ vs. three immune cell surface markers (Tri-color anti-CD3, FITC-anti-CD4 and PE-anti-CD8 or PE-anti-CD56). The profile from one representative donor of seropositive control subjects, (A) gated on lymphocyte cells, and (B) gated on IFN-γ+ cells.